Capping and in vivo toxicity studies of gold nanoparticles

Thi Ha Lien Nghiem, Thi Tuyen Nguyen, Emmanuel Fort, Thanh Phuong Nguyen, Thi My Nhung Hoang, Thi Quy Nguyen and Hong Nhung Tran

Abstract

Water-dispersed colloidal gold nanoparticles (AuNPs) with high concentration were synthesized from metal precursor HAuCl₄. The bovine serum albumin (BSA) and heterobiofunctionalized thiol polyethylene glycol acid (HS–PEG–COOH) were used as biofunctionalized layers for the synthesized AuNPs. The BSA and HS–PEG–COOH bound to the AuNPs were characterized qualitatively and quantitatively by transmission electron microscope and UV-VS spectrophotometer. The fabricated BSA and HS–PEG–COOH-capped AuNPs were introduced in mouse to study its toxicity and its availability in the liver