Chitosan and O-carboxymethyl chitosan modified Fe3O4 for hyperthermic treatment

Thi Thu Trang Mai, Phuong Thu Ha, Hong Nam Pham, Thi Thu Huong Le, Hoai Linh Pham, Thi Bich Hoa Phan, Dai Lam Tran and Xuan Phuc Nguyen

Abstract

In this study magnetic fluids were manufactured by the adsorption of chitosan (CS) and O-carboxymethyl chitosan (OCMCS) on Fe₃O₄nanoparticles to be used as hyperthermic thermoseeds. Fe₃O₄ particles were characterized by physico-chemical methods such as: thermogravimetry analysis (TGA), x-ray diffraction (XRD), Raman spectrum, Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and vibrating sample magnetometer (VSM). The SEM images and XRD patterns showed that the synthesized Fe₃O₄ nanoparticles were of single phase and spherical shape with 10–15 nm in diameter. The VSM measurements showed that Fe₃O₄particles were superparamagnetic with saturation magnetization of 70 emu g⁻¹. The adsorbed layers of CS and OCMCS on the magnetite surface (Fe₃O₄/CS) and (Fe₃O₄/OCMCS) were confirmed by FTIR, Raman spectra and SEM. In the ac magnetic field of 80 Oe and 236 kHz, the saturation heating temperatures of the sample Fe₃O₄/CS and Fe₃O₄/OCMCS were 100 and 98 °C, respectively. At the same concentration of Fe₃O₄ nanoparticles in suspension, the two magnetic fluids exhibited quite high heating capacity, with different behaviors of concentration dependence. The Fe₃O₄/CS and Fe₃O₄/OCMCS nanoparticles would serve as good thermoseeds for localized hyperthermia treatment of cancers