Fluorescence biosensor based on CdTe quantum dots for specific detection of H5N1 avian influenza virus

Thi Hoa Nguyen, Thi Dieu Thuy Ung, Thi Hien Vu, Thi Kim Chi Tran, Van Quyen Dong, Duy Khang Dinh and Quang Liem Nguyen

Abstract

This report highlights the fabrication of fluorescence biosensors based on CdTe quantum dots (QDs) for specific detection of H5N1 avian influenza virus. The core biosensor was composed of (i) the highly luminescent CdTe/CdS QDs, (ii) chromatophores extracted from bacteria Rhodospirillum rubrum, and (iii) the antibody of β-subunit. This core part was linked to the peripheral part of the biosensor via a biotin–streptavidin–biotin bridge and finally connected to the H5N1 antibody to make it ready for detecting H5N1 avian influenza virus. Detailed studies of each constituent were performed showing the image of QDs-labeled chromatophores under optical microscope, proper photoluminescence (PL) spectra of CdTe/CdS QDs, chromatophores and the H5N1 avian influenza viruses