Attaching quantum dots to HER2 specific phage antibodies
Viet Ha Chu, Thi Ha Lien Nghiem, Thi Huyen La, Thi Dieu Thuy Ung, Quang Huan Le, Kim Thuan Tong, Quang Liem Nguyen and Hong Nhung Tran
Abstract
This work presents the results of the attachment of Qdot 655 ITKTM amino (PEG) quantum dots (QDs) (Invitrogen) and CdTe QDs (provided by Institute of Materials Science, VAST) to HER2 (Human Epidermal growth factor Receptor 2) specific phage antibodies (Abs) (provided by Institute of Biotechnology, VAST) in solution. The QDs were attached to the phage display specific HER2 Abs to form a complex QD–Ab. The QDs and complex QD–Ab were characterized by UV-VIS spectroscopy, transmission electron microscopy (TEM) and fluorescence microscopy. The fluorescence images show the QDs conjugated to the phage. Due to the QDs attaching to the surface, the phage dimensions were amplified, so its shape could be observed by optical microscopy. The complex QD–Ab was stable and lasted for a month. The results illustrate the value of the HER2 phage–QD complex as a cancer detection platform
