Synthesis, capping and binding of colloidal gold nanoparticles to proteins

Thi Ha Lien Nghiem, Thi Huyen La, Xuan Hoa Vu, Viet Ha Chu, Thanh Hai Nguyen, Quang Huan Le, Emmanuel Fort, Quang Hoa Do and Hong Nhung Tran

Abstract

Bovine serum albumin (BSA) was used as a stabilizing agent and biofunctionalized layer for water-dispersed gold nanoparticles (NPs) synthesized from metal precursor HAuCl₄. The BSA binding to gold NPs was characterized qualitatively and quantitatively by transmission electron microscopy, UV-VIS and FTIR spectrophotometers. HER2 (human epidermal growth factor receptor 2) specific phage antibodies were attached to BSA stabilized gold NPs to form a gold–antibody complex. An ELISA (enzyme-linked immunosorbent assay) test was done to confirm the bioactivity of antibodies attached to gold NPs