Interaction of nanosilver particles with human lymphocyte cells

Alena Zhornik, Ludmila Baranova, Igor Volotovski, Sergey Chizhik, Elizaveta Drozd, Margarita Sudas, Quoc Buu Ngo, Hoai Chau Nguyen, Thi Ha Huynh and Trong Hien Dao

Abstract

The damaging effects of nanoparticles were hypothesized to be the oxidative stress caused by the formation of reactive oxygen species and initiation of inflammatory reactions. In this context a study on the effects of nanosilver particles on the formation of reactive oxygen species in human lymphocyte culture was carried out. The obtained results showed that fluorescence intensity considerably increased after cells had interacted with nanosilver particles of varying concentrations, indicating the formation of reactive oxygen species and their accumulation in lymphocyte cells. Morphological study of the lymphocyte cells under the effects of nanosilver particles showed that the change in morphology depends on the concentration and size of nanosilver particles: for a size ≤20 nm the lymphocyte cell significantly shrank with pronounced differences in the morphological structure of the cell membrane, but for a size ≥200 nm no change was observed