Electrochemical aptasensor for detecting tetracycline in milk

Thi Hanh Le, Van Phuc Pham, Thi Huyen La, Thi Binh Phan and Quang Huan Le

Abstract

A rapid, simple and sensitive biosensor system for tetracycline detection is very important in food safety. In this paper we developed a label-free aptasensor for electrochemical detection of tetracycline. According to the electrochemical impendence spectroscopy (EIS) analysis, there was a linear relationship between the concentration of tetracycline and the electron transfer resistance from 10 to 3000 ng ml⁻¹ of the tetracycline concentration. The detection limit was 10 ng ml⁻¹ in 15 min detection duration. The prepared aptasensor showed a good reproducibility with an acceptable stability in tetracycline detection. The recoveries of tetracycline in spiked milk samples were in the range of 88.1%–94.2%. The aptasensor has sensitivity 98% and specificity of 100%