Green synthesis of silver nanoparticle and silver based chitosan bionanocomposite using stem extract of Saccharum officinarumand assessment of its antibacterial activity

Kanniah Paulkumar, Gnanadhas Gnanajobitha, Mahendran Vanaja, Manickam Pavunraj and Gurusamy Annadurai

Abstract

Synthesis of nanoparticles and nanocomposites using green route is a major focus of modern nanotechnology. Herein we demonstrate the synthesis of silver nanoparticle and silver based chitosan bionanocomposite using the stem extract of Saccharum officinarum. The absorbance peak at 460 nm in the UV–Vis spectrum reveals the synthesis of silver nanoparticles using the stem extract of Saccharum officinarum. The size of the synthesized silver nanoparticle was in the range of 10–60 nm obtained from transmission electron microscope (TEM) analysis. The presence of silver nanoparticles on the chitosan suspension was identified by scanning electron microscope (SEM) and energy dispersive x-ray spectroscopy (EDS). The presence of possible functional group involved in the reduction of silver metal ions into silver nanoparticles was identified by Fourier transform infrared spectroscopy (FTIR) analysis. The antibacterial activity of the synthesized silver based chitosan bionanocomposite was evaluated against Bacillus subtilis (MTCC 3053), Klebsiella planticola (MTCC 2277), Streptococcus faecalis (ATCC 8043), Pseudomonas aeruginosa (ATCC 9027) and Escherichia coli (ATCC 8739). The antibacterial activity of silver based chitosan bionanocomposite has remarkable scope in medicine, food packaging, textile and pharmaceuticals