Chemically synthesized silver nanoparticles as cell lysis agent for bacterial genomic DNA isolation

Gunajit Goswami, Himangshu Boruah, Trishnamoni Gautom, Dibya Jyoti Hazarika, Madhumita Barooah and Robin Chandra Boro

Abstract

Silver nanoparticles (AgNPs) have seen a recent spurt of use in varied fields of science. In this paper, we showed a novel application of AgNP as a promising microbial cell-lysis agent for genomic DNA isolation. We utilized chemically synthesized AgNPs for lysing bacterial cells to isolate their genomic DNA. The AgNPs efficiently lysed bacterial cells to yield good quality DNA that could be subsequently used for several molecular biology works