Comparative analysis of transition and post transition metal mediated Allamanda cathartica L latex nanoparticles on human peripheral blood mononuclear cells
Kiranmayee P, Prabhudas N, Nandini H S and Kutty A V M
Abstract
The green nanoparticles (NPs) are widely accepted as their synthesis is inexpensive and eco-friendly. In the present study, this approach was applied to synthesise nanoscale particles with transition (copper and zinc) and post-transition (iron) metals and aqueous latex of Allamanda cathartica. The selected metals are safe for human use. The experiments were carried out on human peripheral blood mononuclear cells (hPBMCs) isolated from leftover blood sample collected from diagnostic laboratory. The ratio of latex and metal solutions used to prepare NPs was 3% and 1 millimolar respectively. Ultraviolet-visible, Fourier transmission infra-red, scanning and transmission electron microscopy techniques were used to characterise the NPs. The particles were spherical, hexagonal, aggregated and rectangular and the diameter was ranged from 20.5 to 38.6 nm (zinc oxide), 17.9 to 59.2 nm (copper chloride), 29.2 to 55.4 nm (iron sulphate). Fragmentation and/or shearing took place with all the metal NPs, but clear DNA ladder pattern appeared when hPBMCs were treated with latex ZnO NPs. The order of dead cell percentage was CuCl2 (86%) > FeSO4 (87%) > ZnO (95%) after 72 h of treatment with a concentration of 100 μg. These findings indicate that the synthesised NPs are selectively damaging the DNA. Further studies need to be carried out on cancer cell lines to control the cell proliferation
