In-vitro studies of colloidal silver against SK-mel-28 cancer cell line

Shweta Rajawat and M M Malik

Abstract

In the present research work, synthesis of flavonoid functionalised colloidal silver was done and its morphological as well as in-vitro anti-cancer properties were investigated. Electrodeposition technique is used to prepare colloidal silver solution with black tea extract (2.25% v/v) as capping agent. As-synthesised colloidal silver was characterised using XRD, TEM, UV-visible spectroscopy, FTIR spectroscopy; and anti-cancer properties were investigated using MTT assay. Characterisation results report synthesis of colloidal silver containing pure poly-dispersed spherical silver nanoparticles with an average size of 45 nm and 1.11 ppm concentration. FTIR spectra confirmed interaction between capping agent and silver nanoparticles by presenting shift in transmittance peaks. UV-visible spectra gave 439 nm as characteristic peak value for colloidal silver. IC50 value was obtained as 1.5 μl ml⁻¹. Dose response curve exhibited 72% growth inhibition of cancer cell lines.