Evaluation of bio control potential of AgNPs synthesized from Trichoderma viride

Sambasivam Manikandaselvi, Vembaiyan Sathya, Vellingiri Vadivel, Natarajan Sampath and Pemaiah Brindha

Abstract

Currently, there is a global effort to reduce the use of harmful substances, particularly chemical pesticides in cultivation. The biological mechanism of soil-borne phytopathogens has proven to be a good alternative to the use of chemical pesticides. In recent times, synthesis of nanoparticle from fungus is a trustworthy and sustainable method. The current study explores the synthesis of silver nanoparticles (AgNPs) using Trichoderma viride (T. viride), non-pathogenic fungi isolated from soil. The isolates were confirmed by morphological characters. The cell filtrate of isolates of T. viride was used for the formation of AgNPs from silver nitrate. The synthesised AgNPs were characterised by using UV, XRD, FTIR, SEM and zeta potential analyser. The SEM images indicated that the nanoparticles were spherical and irregular-like in shape and polydispersed in size. XRD analyses were performed to check the crystal-like nature of nanoparticles. Zeta potential study confirms the stability of nanosuspension. In the present study, developed AgNPs of T. viride act as a suitable and persistent biocontrol agent against rice pathogenic fungi such as Rhizoctonia solani and Fusarium moniliforme and provide an improved opportunity for application in crop and disease controlling